ABOUT
ABOUT THE PROCEDURE
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1. Administration of Delta9-tetrahydrocannabinol in mice brain
Administration of Delta9-tetrahydrocannabinol (THC) in 6 mice and vehicle (control) in 6 different mice. Extraction of two aliquotes (T1 and T2) from the THC mice and two other aliquotes (V1 and V2) from the vehicle mice.
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2. Gene expression analysis
Identification of differentially expressed genes. Two different procedures have been used, the individual-wise analysis and the group-wise analysis.
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1. Integration of the mouse interactome
The mouse interactome is generated using the protein-protein interactions integrated from various resources including IntAct, BioGRID, DIP and iRefIndex. To integrate these resources, we used BIANA.
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2. Gene prioritization
We used the genes associated to cannabinoid effects as seeds to rank the genes of the mouse interactome according to the gene-prioritization algorithm from GUILD.
3. Results
We calculated the biological functions significantly enriched in the top 150/250/1000 of the ranking of prioritized genes. We found how the proteasome exerts an important role in the synapsis during cannabinoid exposure.
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